Understanding the technical documentation provided with peptide shipments is essential for verifying product quality and ensuring experimental reproducibility. Certificates of Analysis (COA), HPLC chromatograms, and mass spectrometry data provide critical information about peptide identity, purity, and integrity.
A Certificate of Analysis (COA) is the primary quality document for a peptide batch. It typically includes the peptide sequence, molecular weight, purity (by HPLC), counterion content, and water content. The COA also includes storage recommendations and expiration dates. Reviewing the COA before use helps ensure that the peptide meets your specifications and is suitable for your intended application.
HPLC chromatograms provide information about peptide purity. The chromatogram shows the relative abundance of the main peak (the target peptide) and any impurity peaks. The area under the main peak as a percentage of total peak area represents the purity. A clean chromatogram with a single major peak indicates high purity, while multiple peaks suggest the presence of impurities.
Mass spectrometry data confirms peptide identity. The mass spectrum should show a peak corresponding to the expected molecular weight of the peptide. For modified peptides, the mass spectrum should also show the expected mass shifts corresponding to modifications. Discrepancies between expected and observed mass indicate problems with synthesis or modification.
At PeptideHub, we provide comprehensive analytical documentation with every shipment, including HPLC chromatograms, mass spectra, and detailed COAs. Our commitment to transparency ensures that you have all the information needed to verify product quality and make informed decisions about your research. If you have questions about interpreting our documentation, our technical support team is available to help.