Peptide library synthesis in 96-well and 384-well formats enables high-throughput screening for drug discovery, epitope mapping, and other applications. These high-density formats maximize the number of peptides that can be synthesized and screened in a single experiment, accelerating discovery timelines.
96-well format libraries contain 96 unique peptides, with each well containing a different sequence. This format is compatible with standard laboratory equipment and is widely used for screening applications. 384-well format libraries contain 384 unique peptides, providing four times the capacity of 96-well plates. This higher density enables screening of larger libraries in a single experiment.
Library synthesis in microtiter plates uses automated synthesizers and split-pool methods to produce diverse libraries with high reproducibility. The peptides are synthesized on resin beads or on the plate surface, depending on the application. After synthesis, the peptides are cleaved from the resin and analyzed by mass spectrometry to verify identity and purity.
Applications of peptide libraries include epitope mapping (identifying the minimal sequence recognized by antibodies), drug discovery (screening for compounds that bind to targets), and enzyme substrate identification (identifying sequences cleaved by enzymes). The high-throughput format enables rapid screening of large numbers of peptides, accelerating the identification of active compounds.
At PeptideHub, we offer peptide library synthesis in 96-well and 384-well formats, with high reproducibility and minimal material consumption. Our experienced team can design and synthesize libraries tailored to your specific application, providing the tools needed for high-throughput screening.