Proper storage and handling are essential for maintaining peptide stability and ensuring experimental reproducibility. Peptides are sensitive molecules that can degrade through multiple mechanisms, including hydrolysis, oxidation, and aggregation. Following best practices for storage and handling minimizes degradation and extends shelf life.
Lyophilized peptides should be stored at -20°C or -80°C under desiccation. The low temperature slows chemical reactions, while desiccation prevents hydrolysis. Peptides should be stored in airtight containers with desiccant to prevent moisture absorption. Repeated freeze-thaw cycles should be avoided, as they can cause degradation.
Reconstitution of peptides requires careful attention to solvent selection and handling. The solvent should be appropriate for the peptide's properties, with consideration of solubility and stability. Water, DMSO, or dilute acid/base may be used depending on the peptide. After reconstitution, peptides should be aliquoted to avoid repeated freeze-thaw cycles.
Handling of peptides should minimize exposure to conditions that cause degradation. Peptides should be protected from light, as some modifications are light-sensitive. Exposure to oxygen should be minimized to prevent oxidation. Peptides should be handled at appropriate temperatures, with cold handling for temperature-sensitive peptides.
At PeptideHub, we provide storage recommendations for each peptide based on its specific properties. Our documentation includes storage conditions and shelf life, enabling you to maintain peptide quality throughout its intended use. We also provide guidance on reconstitution and handling to support your experiments.